Identification of RAPD and ISSR markers for drought tolerance in wheat (Triticum aestivum L.)
نویسندگان
چکیده
منابع مشابه
Genetic Diversity of Bread Wheat (Triticum aestivum L.) Genotypes Using RAPD and ISSR Molecular Markers
The importance of grain cultivation especially wheat is obvious in terms of providing human and animal food and its impact on the economy of human societies. The reduction of genetic diversity in cultivars prevents increasing yields in line with rising demand and consumption. Therefore, it is necessary to improve the compatibility of them and increase their genetic extent. In the current, the g...
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Twelve wheat genotypes developed through hybridization programme were screened for genetic diversity through RAPD marker. A total of 102 loci were amplified with 14 primers out of which 91 (89.2%) were polymorphic and only 11(10.8%) were monomorphic. Fragments size ranged from 142bp-5.3kb and fragments produced by various primers ranged from 1-11 with an average of 7.1 fragments per primer. The...
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DNA based molecular markers allow precise, objective and rapid cultivar identification. In this study, five ISSR and two sets of fluorescence-labeled TP-M13-SSR markers were applied for discrimination of eight closely related wheat cultivars. Five ISSR primers revealed a total of 43 distinct reproducible bands, 29 (67.44%) of the 43 bands were polymorphic. The number of polymorphic bands detect...
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In an effort to determine the biochemical markers for identifying genotypes before sowing for drought tolerance, changes in activities of antioxidant enzymes were determined in the seedlings of five drought-tolerant and five drought-sensitive wheat (Triticum aestivum L.) genotypes, each with different genetic background growing under normal and water deficit conditions induced by 6% mannitol. I...
متن کاملPhysiological assessment of drought tolerance in wheat (Triticum aestivum L.) varieties under moisture stress conditions
Drought is one of the major limiting factors in wheat production in Pakistan. Five commercial wheat varieties; Chakwal-97, Inqilab-91, Margalla-99, NR-234 and Wafaq2001 were grown in pots in glasshouse. Plants were subjected to three consecutive drought cycles at tillering, pre-anthesis and milky growth stages by withholding water till leaf rolling started. Measurements of physiological respons...
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ژورنال
عنوان ژورنال: Physiology and Molecular Biology of Plants
سال: 2011
ISSN: 0971-5894,0974-0430
DOI: 10.1007/s12298-011-0096-0